Beschreibung
InhaltsangabePart I: Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology 1. Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun Caixia Gao and Klaus K. Nielsen 2. Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery Shoko Ueki, Shimpei Magori, Benoît Lacroix, and Vitaly Citovsky 3. Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues Mulpuri Sujatha and K.B.R.S. Visarada 4. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun) Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen 5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies Victor Gaba, Moshe Lapidot, and Amit Gal-On 6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn Part II: Biolistic DNA Delivery in Nematodes 7. Biolistic Transformation of Caenorhabditis elegans Meltem Isik and Eugene Berezikov 8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher 9. Biolistic Transformation of Brugia malayi Tarig B. Higazi and Thomas R. Unnasch Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro 10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens 11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang 12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida 13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O'Brien and Sarah C.R. Lummis Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo 14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai 15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili 16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo 17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz 18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus Part V: Biolistic DNA Vaccination in Animal Disease Models 19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller 20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin 21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitne