Beschreibung
Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches.
Autorenportrait
Inhaltsangabe1. Measuring Freezing Tolerance: Survival and Regrowth Assays Daniel Z. Skinner and Kimberly Garland-Campbell 2. Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays Anja Thalhammer, Dirk K. Hincha and Ellen Zuther 3. Conducting Field Trials for Freezing Tolerance Breeding in Cereals Luigi Cattivelli 4. A Whole Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance Annick Bertrand, Yves Castonguay and Josée Bourassa 5. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation Evelyne Téoulé and Carine Géry 6. Common Garden Experiments to Characterize Cold Acclimation Responses in Plants from Different Climatic RegionsAndrey Malyshev, Hugh A. L. Henry and Juergen Kreyling 7. Identification of Arabidopsis Mutants with Altered Freezing Tolerance Carlos Perea-Resa and Julio Salinas 8. Infrared Thermal Analysis of Plant Freezing Processes Gilbert Neuner and Edith Kuprian 9. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues Seizo Fujikawa and Keita Endoh 10. Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images David P. Livingston III and Tan D. Tuong 11. Proteomic Approaches to Identify Cold Regulated Soluble Proteins Stefanie Döll, Rico Lippmann and Hans-Peter Mock 12. Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura and Matsuo Uemura 13. Profiling Methods to Identify Cold Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry Frederik Dethloff, Alexander Erban, Isabel Orf, Jessica Alpers, Ines Fehrle, Olga Beine-Golovchuk, Stefanie Schmidt, Jens Schwachtje and Joachim Kopka 14. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid CompositionHieu Sy Vu, Sunitha Shiva, Aaron Smalter Hall and Ruth Welti 15. Quantification of Superoxide and Hydrogen Peroxide in LeavesIlona Juszczak and Margarete Baier 16. Estimating Ice Encasement Tolerance of Herbage Plants Bjarni E. Gudleifsson and Brynhildur Bjarnadottir 17. Characterization of Ice Binding Proteins from Sea Ice Algae Maddalena Bayer-Giraldi, EonSeon Jin and Peter W. Wilson 18. Isolation and Characterization of Ice Binding Proteins from Higher Plants Adam J. Middleton, Barbara Vanderbeld, Melissa Bredow, Heather Tomalty, Peter L. Davies and Virginia K. Walker
